bacD [2017-8-11 16:0:3]

alanine-anticapsin ligase

Locus: BSU37710

Isoelectric point: 4.68

Molecular weight: 52.10 kDa

Protein length: 472 aa

Gene length: 1416 bp

Function: biosynthesis of the antibiotic bacilysin

Product: alanine-anticapsin ligase

Essential: no

E.C.: 6.3.2.28

Synonyms: ywfE, ipa-83d

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Miscellaneous metabolic pathways → Biosynthesis of antibacterial compounds
• Lifestyles → Coping with stress → Biosynthesis of antibacterial compounds

Gene

Coordinates: 3,870,668 → 3,872,086

Phenotypes of a mutant

• a bacD point mutation has been isolated upon selction at low pressure PubMed

The protein

Catalyzed reaction/ biological activity

• ligation of anticapsin to L-Ala to form bacilysin, ultimate step in bacilysin biosynthesis PubMed

Structure

• 3VMM PubMed

Expression and Regulation

Operons

• Genes: bacA-bacB-bacC-bacD-bacE-bacF
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CodY: repression, PubMed, in CodY regulon
  • AbrB: repression, PubMed, in AbrB regulon
  • ScoC: repression, PubMed, in ScoC regulon

  Regulation

  • expression of the operon is strongly induced at the beginning of biofilm formation PubMed

Biological materials

Mutant

• MGNA-B241 (ywfE::erm), available at the NBRP B. subtilis, Japan

References

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